Generator

Part:BBa_K361002:Design

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM10_Hong_Kong-CUHK   (2010-10-24)


Rci recombinase system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 362
    Illegal NgoMIV site found at 857
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the most optimal system for Rci site-specific recombinase production.


Source

The promotor is come from lacI regulated one: BBa_R0010 The ribosome binding site is come from the defines RBS efficiency equal to one : BBa_B0034 The terminator is come from a doble terminator with bidirectional termination: BBa_B0014

References